[
    {
        "id": "authors:9f7kk-0vg03",
        "collection": "authors",
        "collection_id": "9f7kk-0vg03",
        "cite_using_url": "https://authors.library.caltech.edu/records/9f7kk-0vg03",
        "type": "article",
        "title": "Carbon monoxide oxidation expands the known metabolic capacity in anaerobic methanotrophic consortia",
        "author": [
            {
                "family_name": "Guo",
                "given_name": "Yongzhao",
                "orcid": "0009-0005-3983-8382",
                "clpid": "Guo-Yongzhao"
            },
            {
                "family_name": "Utter",
                "given_name": "Daniel R.",
                "orcid": "0000-0003-3322-7108",
                "clpid": "Utter-Daniel-R"
            },
            {
                "family_name": "Murali",
                "given_name": "Ranjani",
                "orcid": "0000-0003-4073-9910",
                "clpid": "Murali-Ranjani"
            },
            {
                "family_name": "Orphan",
                "given_name": "Victoria J.",
                "orcid": "0000-0002-5374-6178",
                "clpid": "Orphan-V-J"
            }
        ],
        "abstract": "Consortia of anaerobic methane-oxidizing archaea (ANME-2) and sulphate-reducing bacteria (SRB) represent globally relevant syntrophic associations capable of growing with minimal amounts of free energy and can persist when methane becomes limiting. Carbon monoxide (CO) has been reported in seep environments and represents a thermodynamically favourable alternative electron donor due to its low reduction potential. Here, we show that environmental ANME-SRB consortia can oxidize CO in the absence of methane, in anoxic microcosm experiments using a combination of stable isotope geochemical tracers, metatranscriptomics, and single cell activity measurements (FISH\u2013nanoSIMS). The oxidation of CO was coupled with sulphate-reduction by syntrophic consortia, and, in the absence of sulphate, through CO2 reduction to methane by ANME-2. Under these conditions, the production of methane was one ninth the rate of methanotrophy coupled to sulphate-reduction. Paired single cell FISH-nanoSIMS analysis of anabolic activity indicates that CO respiration appears to support cell maintenance rather than active growth, consistent with the observed down-regulation of energy generating complexes in ANME (e.g., mtr, rnf, etc.). The versatile capability of CO oxidation by anaerobic methanotrophic consortia broadens our understanding of carbon cycling in methane seeps and highlights potential mechanisms of resilience by methanotrophic archaea under changing geochemical regimes.",
        "doi": "10.1038/s41467-026-71433-9",
        "pmcid": "PMC13079720",
        "issn": "2041-1723",
        "publisher": "Nature Publishing Group",
        "publication": "Nature Communications",
        "publication_date": "2026-04-14",
        "series_number": "1",
        "volume": "17",
        "issue": "1",
        "pages": "3461"
    },
    {
        "id": "authors:bz6mp-4wh10",
        "collection": "authors",
        "collection_id": "bz6mp-4wh10",
        "cite_using_url": "https://authors.library.caltech.edu/records/bz6mp-4wh10",
        "type": "article",
        "title": "Methane-powered sea spiders: Diverse, epibiotic methanotrophs serve as a source of nutrition for deep-sea methane seep Sericosura",
        "author": [
            {
                "family_name": "Dal B\u00f3",
                "given_name": "Bianca",
                "orcid": "0009-0007-1691-8181"
            },
            {
                "family_name": "Guo",
                "given_name": "Yongzhao",
                "orcid": "0009-0005-3983-8382",
                "clpid": "Guo-Yongzhao"
            },
            {
                "family_name": "Mayr",
                "given_name": "Magdalena J.",
                "orcid": "0000-0002-3182-1480",
                "clpid": "Mayr-Magdalena-J"
            },
            {
                "family_name": "Pereira",
                "given_name": "Olivia S."
            },
            {
                "family_name": "Levin",
                "given_name": "Lisa A.",
                "orcid": "0000-0002-2858-8622"
            },
            {
                "family_name": "Orphan",
                "given_name": "Victoria J.",
                "orcid": "0000-0002-5374-6178",
                "clpid": "Orphan-V-J"
            },
            {
                "family_name": "Goffredi",
                "given_name": "Shana K.",
                "orcid": "0000-0002-9110-9591"
            }
        ],
        "abstract": "<p>Methane seeps harbor uncharacterized animal&ndash;microbe symbioses with unique nutritional strategies. Three undescribed sea spider species (family Ammotheidae; genus&nbsp;<em>Sericosura</em>) endemic to methane seeps were found along the eastern Pacific margin, from California to Alaska, hosting diverse methane- and methanol-oxidizing bacteria on their exoskeleton. &delta;<sup>13</sup>C tissue isotope values of in situ specimens corroborated methane assimilation (&minus;45&permil;, on average). Live animal incubations with&nbsp;<sup>13</sup>C-labeled methane and methanol, followed by nanoscale secondary ion mass spectrometry, confirmed that carbon derived from both compounds was actively incorporated into the tissues within five days. Methano- and methylotrophs of the bacterial families Methylomonadaceae, Methylophagaceae and Methylophilaceae were abundant, based on environmental metagenomics and 16S rRNA sequencing, and fluorescence and electron microscopy confirmed dense epibiont aggregations on the sea spider exoskeleton. Egg sacs carried by the males hosted identical microbes suggesting vertical transmission. We propose that these sea spiders farm and feed on methanotrophic and methylotrophic bacteria, expanding the realm of animals known to harness C1 compounds as a carbon source. These findings advance our understanding of the biology of an understudied animal lineage, unlocking some of the unique nutritional links between the microbial and faunal food webs in the oceans.</p>",
        "doi": "10.1073/pnas.2501422122",
        "issn": "0027-8424",
        "publisher": "National Academy of Sciences",
        "publication": "Proceedings of the National Academy of Sciences",
        "publication_date": "2025-06-16",
        "series_number": "26",
        "volume": "122",
        "issue": "26",
        "pages": "e2501422122"
    }
]