[
    {
        "id": "thesis:18529",
        "collection": "thesis",
        "collection_id": "18529",
        "cite_using_url": "https://resolver.caltech.edu/CaltechTHESIS:04302026-160335841",
        "type": "thesis",
        "title": "I. Methods for Restriction Endonuclease Studies of DNA Structure. II. Restriction Endonucleolytic Characterization of Animal Mitochondrial DNAs and Human Globin Genes",
        "author": [
            {
                "family_name": "Parker",
                "given_name": "Richard Carl",
                "clpid": "Parker-Richard-Carl"
            }
        ],
        "thesis_advisor": [
            {
                "family_name": "Vinograd",
                "given_name": "Jerome Rubin",
                "clpid": "Vinograd-J"
            },
            {
                "family_name": "Baldeschwieler",
                "given_name": "John D.",
                "clpid": "Baldeschwieler-J-D"
            },
            {
                "family_name": "Maniatis",
                "given_name": "Tom",
                "orcid": "0000-0002-2722-8633",
                "clpid": "Maniatis-T"
            }
        ],
        "thesis_committee": [
            {
                "family_name": "Unknown",
                "given_name": "Unknown"
            }
        ],
        "local_group": [
            {
                "literal": "div_biol"
            }
        ],
        "abstract": "<p>An initial approach to the structural organization of DNA is restriction\r\nendonuclease site mapping and gel electrophoretic analysis. This is true for genomes\r\nof the simplest or greatest complexities.</p>\r\n\r\n<p>Two techniques are presented in this thesis that facilitate this approach.\r\nThe first uses ethidium bromide to limit the action of a restriction endonuclease\r\non a closed circular DNA in order to derive a set of circularly permuted linear\r\nmolecules. These molecules, after appropriate treatment, can be used to orient\r\nthe restriction endonuclease sites and to calibrate the relationship between\r\nelectrophoretic mobility and DNA fragment size without the introduction of\r\nexternal standards.</p>\r\n\r\n<p>The second technique utilizes a low melting temperature agarose. It\r\nprovides a simple system for two-dimensional electrophoretic analysis of DNA\r\nmolecules with restriction endonuclease digestion of the DNA occurring after\r\nthe first electrophoretic separation and before the second.</p>\r\n\r\n<p>These techniques and others were used to study mitochondrial DNA from\r\nmice and rats. Some of these data explore the evolutionary divergence of the\r\nmtDNA in these animals. This information can be compared to evolutionary\r\nstudies with nuclear DNA.</p>\r\n\r\n<p>Additionally, chromosomal DNA from patients with normal hemoglobin\r\nand hemoglobin Lepore was studied. Using this type of analysis we were able\r\nto demonstrate that a change in the amino acid sequence of some of the \u03b2-related\r\nglobin chains in hemoglobin Lepore is associated with a change in DNA structure.</p>",
        "doi": "10.7907/pphq-jv67",
        "publication_date": "1979",
        "thesis_type": "phd",
        "thesis_year": "1979"
    }
]