[
    {
        "id": "thesis:18598",
        "collection": "thesis",
        "collection_id": "18598",
        "cite_using_url": "https://resolver.caltech.edu/CaltechTHESIS:05192026-175558424",
        "primary_object_url": {
            "basename": "Stitt_BL_1978.pdf",
            "content": "final",
            "filesize": 77869893,
            "license": "other",
            "mime_type": "application/pdf",
            "url": "/18598/1/Stitt_BL_1978.pdf",
            "version": "v2.0.0"
        },
        "type": "thesis",
        "title": "Role of the Host Cell in Bacteriophage T4 Development",
        "author": [
            {
                "family_name": "Stitt",
                "given_name": "Barbara Landale",
                "clpid": "Stitt-Barbara-Landale"
            }
        ],
        "thesis_advisor": [
            {
                "family_name": "Wood",
                "given_name": "William Barry",
                "clpid": "Wood-W-B"
            }
        ],
        "thesis_committee": [
            {
                "family_name": "Unknown",
                "given_name": "Unknown"
            }
        ],
        "local_group": [
            {
                "literal": "div_biol"
            }
        ],
        "abstract": "<p>More than six hundred mutant host-defective bacteria were selected\r\nthat were able to adsorb and be killed by phage T4<sup>+</sup> but were unable to support\r\nits growth. The mutants were categorized into seven classes and representatives\r\nwere screened to determine the nature of the host block. The majority of the\r\nmutants clearly blocked in phage assembly interfered with T4<sup>+</sup> capsid formation\r\nat the very early level of gene 31 function. gp31 acts at or before head core\r\nformation, which precedes the assembly of proheads and the formation of the\r\ncapsid proper. Mutant phage able to overcome the host block carried mutations\r\nin either gene 31 or in the gene for the major capsid protein. The host mutations\r\nall mapped near purA, at two or possibly three different sites.</p>\r\n\r\n<p>A host mutant class that only marginally passed our screens for assembly-related\r\nmutants had a pleiotropic effect on T4<sup>+</sup> growth: specific, unidentified\r\nearly phage proteins were made in reduced quantity or not at all; phage DNA\r\nsynthesis was depressed 50%; specific late, structural phage proteins including\r\nsome tail-baseplate and phage collar components were reduced or missing, and\r\nhost cell lysis was delayed and slow. Phage that could overcome this block mapped\r\nin functionally undefined regions of the T4 genome, most near gene 39. The\r\nhost mutation mapped to within 0.5 minutes of nalA</p>\r\n\r\n<p>Possible mechanisms of action for the various host mutations are discussed.</p>",
        "doi": "10.7907/aez8-c005",
        "publication_date": "1978",
        "thesis_type": "phd",
        "thesis_year": "1978"
    }
]